abstract：:Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer's disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed i...

journal_title：International journal of molecular sciences

authors： Pogue AI,Jones BM,Bhattacharjee S,Percy ME,Zhao Y,Lukiw WJ

更新日期：2012-01-01 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) are currently the marker of choice in a variety of genetic studies. Using the high resolution melting (HRM) genotyping approach, 101 gene-based SNP markers were developed for Apostichopus japonicus, a sea cucumber species with economic significance for the aquaculture industry in...

journal_title：International journal of molecular sciences

authors： Du H,Bao Z,Yan J,Tian M,Mu X,Wang S,Lu W

更新日期：2012-01-01 00:00:00

abstract：:MnTE-2-PyP, a superoxide dismutase mimetic, inhibited OVA-induced airway inflammation in mice suggesting an effect on Th2 responsiveness. Thus, we hypothesized that MnTE-2-PyP may alter dendritic cell-Th2 interactions. Bone marrow derived dendritic cells (DC) and OVA(323-339)-specific Th2 cells were cultured separatel...

journal_title：International journal of molecular sciences

authors： Jungsuwadee P,Weaver MR,Gally F,Oberley-Deegan RE

更新日期：2012-01-01 00:00:00

abstract：:Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The es...

journal_title：International journal of molecular sciences

authors： Xin JY,Wang Y,Liu T,Lin K,Chang L,Xia CG

更新日期：2012-01-01 00:00:00

abstract：:CA125 is the most widely used tumour marker in ovarian cancer with unsatisfactory sensitivity and specificity especially at early stage. It is quantified by antibody-based immunoassays; however different molecular weight isoforms have been described in the literature which have never been validated by mass spectrometr...

journal_title：International journal of molecular sciences

authors： Weiland F,Fritz K,Oehler MK,Hoffmann P

更新日期：2012-01-01 00:00:00

abstract：:Considering the undesirable attributes of synthetic fungicides and the availability of Ficus species in China, the stem of Ficus tikoua Bur. was investigated. One new antifungal pyranoisoflavone, 5,3',4'-trihydroxy-2″,2″-dimethylpyrano (5″,6″:7,8) isoflavone (1), together with two known isoflavones, wighteone (2) and ...

journal_title：International journal of molecular sciences

authors： Wei S,Wu W,Ji Z

更新日期：2012-01-01 00:00:00

abstract：:C-type lectin receptors and their adaptor molecules are involved in the recognition of glycosylated self-antigens and pathogens. However, little is known about the species- and organ-specific expression profiles of these molecules. We therefore determined the mRNA expression levels of Dectin-1, MR1, MR2, DC-SIGN, Syk,...

journal_title：International journal of molecular sciences

authors： Lech M,Susanti HE,Römmele C,Gröbmayr R,Günthner R,Anders HJ

更新日期：2012-01-01 00:00:00

abstract：:ALG-2 is a penta-EF-hand Ca(2+)-binding protein and interacts with a variety of intracellular proteins. Two types of ALG-2-binding motifs have been determined: type 1, PXYPXnYP (X, variable; n = 4), in ALIX and PLSCR3; type 2, PXPGF, in Sec31A and PLSCR3. The previously solved X-ray crystal structure of the complex be...

journal_title：International journal of molecular sciences

authors： Takahashi T,Suzuki H,Inuzuka T,Shibata H,Maki M

更新日期：2012-01-01 00:00:00

abstract：:Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the cor...

journal_title：International journal of molecular sciences

authors： Costa MC,Leitão AL,Enguita FJ

更新日期：2012-01-01 00:00:00

abstract：:Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal and differentiation of embryonic stem cell. The aim of this study was to detect the expression of Sox2 and Oct4 and analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression of Sox2 and Oct4 we...

journal_title：International journal of molecular sciences

authors： Li X,Wang J,Xu Z,Ahmad A,Li E,Wang Y,Qin S,Wang Q

更新日期：2012-01-01 00:00:00

abstract：:Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...

journal_title：International journal of molecular sciences

authors： Kuczyńska A,Kosmala A,Surma M,Adamski T

更新日期：2012-01-01 00:00:00

abstract：:We report molecular dynamics simulations of confined liquid crystals using the Gay-Berne-Kihara model. Upon isobaric cooling, the standard sequence of isotropic-nematic-smectic A phase transitions is found. Upon further cooling a reentrant nematic phase occurs. We investigate the temperature dependence of the self-dif...

journal_title：International journal of molecular sciences

authors： Stieger T,Mazza MG,Schoen M

更新日期：2012-01-01 00:00:00

abstract：:The ovarian cancer biomarker CA125 has been extensively investigated over the last 30 years. The knowledge about the exact molecular nature of this protein, however, remains fragmented. This review provides an overview of the structural research regarding CA125, and presents an orthogonal verification method to confir...

journal_title：International journal of molecular sciences

authors： Weiland F,Martin K,Oehler MK,Hoffmann P

更新日期：2012-01-01 00:00:00

abstract：:The activation of extracellular signal-regulated kinases (ERK) leads to a number of cellular changes associated with the development of long-term memory. Using cultured cortical neurons, we previously showed that the n-hexane extract prepared from the peels of Citrus grandis (Kawachi bankan) induces the activation of ...

journal_title：International journal of molecular sciences

authors： Furukawa Y,Watanabe S,Okuyama S,Nakajima M

更新日期：2012-01-01 00:00:00

abstract：:CZ48, chemically camptothecin-20-O-propionate hydrate, is currently under clinical investigation. The kinetics of the metabolite camptothecin (CPT) formation and of CZ48 depletion in mouse and human liver microsomes in the presence or absence of NADPH was examined. The formation rate of camptothecin in human liver mic...

journal_title：International journal of molecular sciences

authors： Liu X,Dejesus A,Cao Z,Vardeman D,Giovanella B

更新日期：2012-01-01 00:00:00

abstract：:Vascular inflammation plays a key role in the pathogenesis and progression of atherosclerosis, a main complication of diabetes. The present study investigated whether an aqueous extract of Portulaca oleracea (AP) prevents the TNF-α-induced vascular inflammatory process in the human umbilical vein endothelial cell (HUV...

journal_title：International journal of molecular sciences

authors： Lee AS,Kim JS,Lee YJ,Kang DG,Lee HS

更新日期：2012-01-01 00:00:00

abstract：:Heat shock proteins (HSPs) play various stress-protective roles in plants. In this study, three HSP genes were isolated from a suppression subtractive hybridization (SSH) cDNA library of Ginkgo biloba leaves treated with cold stress. Based on the molecular weight, the three genes were designated GbHSP16.8, GbHSP17 and...

journal_title：International journal of molecular sciences

authors： Cao F,Cheng H,Cheng S,Li L,Xu F,Yu W,Yuan H

更新日期：2012-01-01 00:00:00

abstract：:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families...

journal_title：International journal of molecular sciences

authors： Xi L,Ruan J,Huang Y

更新日期：2012-01-01 00:00:00

abstract：:Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-gl...

journal_title：International journal of molecular sciences

authors： Hsu FL,Huang WJ,Wu TH,Lee MH,Chen LC,Lu HJ,Hou WC,Lin MH

更新日期：2012-01-01 00:00:00

abstract：:The purpose of the study was to investigate the antioxidant characteristics of Anisomeles indica methanol extract and the inhibitory effect of ovatodiolide on melanogenesis. In the study, the antioxidant capacities of A. indica methanol extract such as DPPH assay, ABTS radical scavenging assay, reducing capacity and m...

journal_title：International journal of molecular sciences

authors： Huang HC,Lien HM,Ke HJ,Chang LL,Chen CC,Chang TM

更新日期：2012-01-01 00:00:00

abstract：:Gelam honey exerts anti-inflammatory and antioxidant activities and is thought to have potent effects in reducing infections and healing wounds. The aim of this study was to investigate the effects of intravenously-injected Gelam honey in protecting organs from lethal doses of lipopolysaccharide (LPS). Six groups of r...

journal_title：International journal of molecular sciences

authors： Kassim M,Mansor M,Al-Abd N,Yusoff KM

更新日期：2012-01-01 00:00:00

abstract：:A field experiment was conducted using a 3 × 3 orthogonal regression design to explore the growth promotion of one-year-old Yunnan pine seedlings (Pinus yunnanensis Franch.) in response to foliar application of IAA (indole-3-acetic acid) at rates of 0, 200 and 400 mg·L(-1) and IBA (indole-3-butyric acid) at rates of 0...

journal_title：International journal of molecular sciences

authors： Xu Y,Zhang Y,Li Y,Li G,Liu D,Zhao M,Cai N

更新日期：2012-01-01 00:00:00

abstract：:Genistein, daidzein, glycitein and quercetin are flavonoids present in soybean and other vegetables in high amounts. These flavonoids can be metabolically converted to more active forms, which may react with guanine in the DNA to form complexes and can lead to DNA depurination. We assumed two ultimate carcinogen forms...

journal_title：International journal of molecular sciences

authors： Abdallah HH,Mavri J,Repič M,Lee VS,Wahab HA

更新日期：2012-01-01 00:00:00

abstract：:Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild ind...

journal_title：International journal of molecular sciences

authors： Li R,Yang J,Yang J,Dao Z

更新日期：2012-01-01 00:00:00

abstract：:Sabicea species are used in the Amazon for treatment of fever and malaria, which suggests that its chemical constituents may have some effect on pain and inflammation. Phytochemical analysis of the hexane fraction obtained from the crude ethanol extract from Sabicea grisea var. grisea Cham. & Schltdl (Rubiaceae), an e...

journal_title：International journal of molecular sciences

authors： de Oliveira AM,Conserva LM,de Souza Ferro JN,de Almeida Brito F,Lyra Lemos RP,Barreto E

更新日期：2012-01-01 00:00:00

abstract：:A psychrotolerant bacterium, strain T-3 (identified as Psychrobacter piscatorii), that exhibited an extraordinarily high catalase activity was isolated from the drain pool of a plant that uses H(2)O(2) as a bleaching agent. Its cell extract exhibited a catalase activity (19,700 U·mg protein(-1)) that was higher than t...

journal_title：International journal of molecular sciences

authors： Kimoto H,Yoshimune K,Matsuyma H,Yumoto I

更新日期：2012-01-01 00:00:00

abstract：:The conserved serine/threonine kinase mTOR (the mammalian target of rapamycin), a downstream effector of the PI3K/AKT pathway, forms two distinct multiprotein complexes: mTORC1 and mTORC2. mTORC1 is sensitive to rapamycin, activates S6K1 and 4EBP1, which are involved in mRNA translation. It is activated by diverse sti...

journal_title：International journal of molecular sciences

authors： Pópulo H,Lopes JM,Soares P

更新日期：2012-01-01 00:00:00

abstract：:Antioxidants are among the most popular health-protecting products, sold worldwide without prescription. Indeed, there are many reports showing the benefits of antioxidants but only a few questioning the possible harmful effects of these "drugs". The normal balance between antioxidants and free radicals in the body is...

journal_title：International journal of molecular sciences

authors： Villanueva C,Kross RD

更新日期：2012-01-01 00:00:00

abstract：:Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure p...

journal_title：International journal of molecular sciences

authors： Mendonça L,Hache F

更新日期：2012-01-01 00:00:00

abstract：:Kinase insert domain receptor (KDR) inhibitors have been proved to be very effective anticancer agents. Molecular docking, 3D-QSAR methods, CoMFA and CoMSIA were performed on pyrrolo[3,2-d]pyrimidine derivatives as non-ATP competitive KDR inhibitors (type II). The bioactive conformation was explored by docking one pot...

journal_title：International journal of molecular sciences

authors： Wu XY,Chen WH,Wu SG,Tian YX,Zhang JJ

更新日期：2012-01-01 00:00:00

abstract：:The aim of this study was to explore the optimal therapeutic dose and time window of picroside II for treating cerebral ischemic injury in rats according to the orthogonal test. The middle cerebral artery occlusion (MCAO) models were established by intraluminally inserting a thread into middle cerebral artery (MCA) fr...

journal_title：International journal of molecular sciences

authors： Pei H,Su X,Zhao L,Li H,Guo Y,Zhang M,Xin H

更新日期：2012-01-01 00:00:00

abstract：:(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western bl...

journal_title：International journal of molecular sciences

authors： Pan L,Hu JL,Wang WJ,Zhang XJ,Wei J,Liu ZD,Zhang YH,Xu HM

更新日期：2012-01-01 00:00:00

abstract：:The western flower thrips, Frankliniella occidentalis (Pergande), is an invasive species and the most economically important pest within the insect order Thysanoptera. For a better understanding of the genetic makeup and migration patterns of F. occidentalis throughout the world, we characterized 18 novel polymorphic ...

journal_title：International journal of molecular sciences

authors： Yang XM,Sun JT,Xue XF,Zhu WC,Hong XY

更新日期：2012-01-01 00:00:00

abstract：:Supercritical fluid extraction (SFE) was used in the analysis of bacterial respiratory quinone (RQ), bacterial phospholipid fatty acid (PLFA), and archaeal phospholipid ether lipid (PLEL) from anaerobically digested sludge. Bacterial RQ were determined using ultra performance liquid chromatography (UPLC). Determinatio...

journal_title：International journal of molecular sciences

authors： Hanif M,Atsuta Y,Fujie K,Daimon H

更新日期：2012-01-01 00:00:00

abstract：:Circular dichroism (CD) is a useful technique for monitoring changes in the conformation of antimicrobial peptides or gelatin. In this study, interactions between cationic peptides and gelatin were observed without affecting the triple helical content of the gelatin, which was more strongly affected by anionic surfact...

journal_title：International journal of molecular sciences

authors： Gopal R,Park JS,Seo CH,Park Y

更新日期：2012-01-01 00:00:00

abstract：:The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, ...

journal_title：International journal of molecular sciences

authors： Wu CC,Li XB,Han TS,Li P,Liu GW,Wang WZ,Wang Z

更新日期：2012-01-01 00:00:00

abstract：:The only curative treatment for hepatic failure is liver transplantation. Unfortunately, this treatment has several major limitations, as for example donor organ shortage. A previous report demonstrated that transplantation of induced pluripotent stem cells without reprogramming factor c-Myc (3-genes iPSCs) attenuates...

journal_title：International journal of molecular sciences

authors： Chang HM,Liao YW,Chiang CH,Chen YJ,Lai YH,Chang YL,Chen HL,Jeng SY,Hsieh JH,Peng CH,Li HY,Chien Y,Chen SY,Chen LK,Huo TI

更新日期：2012-01-01 00:00:00

abstract：:Peroxiredoxins (Prxs) are a recently discovered family of antioxidant enzymes that catalyze the reduction of peroxides and alkyl peroxides. In this study, four Prx genes (named as ThPrxII, ThPrxIIE, ThPrxIIF, and Th2CysPrx) were cloned from Tamarix hispida. Their expression profiles in response to stimulus of NaCl, Na...

journal_title：International journal of molecular sciences

authors： Gao C,Zhang K,Yang G,Wang Y

更新日期：2012-01-01 00:00:00

abstract：:Microbial fuel cells (MFCs) represent a novel technology for wastewater treatment with electricity production. Electricity generation with simultaneous nitrate reduction in a single-chamber MFC without air cathode was studied, using glucose (1 mM) as the carbon source and nitrate (1 mM) as the final electron acceptor ...

journal_title：International journal of molecular sciences

authors： Nimje VR,Chen CC,Chen HR,Chen CY,Tseng MJ,Cheng KC,Shih RC,Chang YF

更新日期：2012-01-01 00:00:00

abstract：:A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced th...

journal_title：International journal of molecular sciences

authors： Hou X,Shen Y,Zhang C,Zhang L,Qin Y,Yu Y,Wang L,Sun X

更新日期：2012-01-01 00:00:00